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Data acquisition and image processing The purified tirzepatideGIPRG s Nb35 complex at a concentration of 18-20 mg/mL was mixed with 100 M tirzepatide at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific)

Acknowledgements A special thanks to all the children participating in the original study and to great colleagues Nina Wittorff Jensen, Susanne Zederkopff, Charlotte Gade Farcinsen Leth, Rebekka Gerwig, Camilla Ss Nielsen and Srine Birkelund from Steno Laboratorium, Department of Diabetes Treatment, Steno Diabetes Center Copenhagen for helping with the MSD analysis

Mehrzadi, S., Karimi, M