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Upon 85% confluence, PBEC were starved overnight using Lonza Bronchial Epithelial Basal Medium supplemented with Bronchial Epithelial Cell Growth Medium singlequots (except gentamycin, bovine pituitary extract and epidermal growth factor) (Lonza), BSA (1.5 g/mL) and 1% penicillin/streptomycin (Gibco) (i.e., BEGM starvation)

Canad The Framework Convention on Tobacco Controls (FCTC) guiding principle is that "every person should be informed of the health consequences, addictive nature, and mortal threat posed by tobacco consumption and exposure to tobacco smoke." 1 Indeed, smokers who are more aware of these risks are more motivated to quit smoking

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