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Like taking the edge off, gently

Following three 10 min washes in PBS-T, the membrane was incubated with HRP conjugated secondary antibodies, goat anti-rabbit (Thermo Scientific, 656120, 1:5000 dilution), or goat anti-mouse IgG antibody (Thermo Scientific, 626520, 1:5000 dilution) in blocking buffer for 1 hour at room temperature

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Additional Information Packing: 14 cigarettes per pack Aroma: Natural tobacco with mild sweet, hay-like undertones Lenght: 84 mm Size: King size Tar: 6 mg Nicotine: 0.7 mg Cigarette Type: Filtered cigarette Origin: USA Produced by: Santa Fe Natural Tobacco Company