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Nicotine exposure during this time disrupts normal developmental trajectories, altering neurotransmitter systems (especially dopamine and serotonin) and leading to lasting changes in brain structure and function (, )

The specific parameters for analysis of each of these four subsets that differ from those above are as follows: (1) KRT8 high /secretory cells: Dataset integration was carried out with the top 3000 genes, UMAP was created with n.neighbors = 50, min.dist = 0.3, and SNN clustering was done using the Louvain algorithm, k.param = 50, and resolution = 0.54