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Aliquots of 100 L were withdrawn, diluted 1:100 with methanol, and filtered with a syringe-mounted PVDF (polyvinylidene fluoride) filter, 13 mm diameter, 0.22 m pore size, obtained from Thermo Fisher Scientific, Inc
This implies that the lower soil nutrient levels were not due to deficiency, but rather driven by the vigorous root system which accelerated the active uptake of N and K, effectively transferring them from the soil pool to the plant biomass
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