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Prior to sacrificing the rats, the ME effusion and tympanic membrane statuses were confirmed using a model AM4113EUT (R4) portable digital microscope (USB) (Dino-Lite, New Taipei City, Taiwan)

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Acknowledgements This research was supported by a research grant (PE23905-360-05) funded by Polar Academic Program (PAP) from the Korea Polar Research Institute (KOPRI) and a research grant (PE25100) from KOPRI