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Despite the intracellular abundance of exogenously supplied 13 C-creatine or 13 C-creatinine, only 13 C-creatinine, and not 13 C-creatine, resulted in enrichment of the heavy carbon label within CR (Figure 3, C and D), indicating that creatinine was the precursor for CR
The expression construct (Joensuu et al., 2010) included CaMV35S promoter and signal sequence to target GFP-HFBI expression to secretion pathway
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