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Sequencing of Prepared Library The Ampure beads (Beckman Coulter Inc., Indianapolis, IN, United States) were used to purify the amplicons of each sample by eliminating the unused primers and to prepare the sequencing libraries an additional 8 cycles of PCR was executed via Illumina barcoded adapters (Supplementary Table 1)

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It's a creepy story, far creepier than some of the season's other monster or ghost-themed episodes, including its own take on Night of the Living Dead